Rosa Di Liddo
Department of Pharmaceutical and Pharmacological Sciences, University of Padova
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Short CV
1995: Degree in Biological Sciences, University of Bari, Italy
2001: Research Fellowship in "Automatic sequencing and spectratyping of tumors and leukemias” at Gozzadini Pediatric Clinic -Sant'Orsola Hospital-Bologna, Italy
2002-2004: PhD course in Tissue Engineering and Transplants, Dept of Pharmaceutical Sciences, University of Padova, Italy
2005-2007: Post-Doctoral Research Fellowship in “Cardiovascular complications of obesity: study of the interactions between leptin and regulatory peptides (ETADM) during adipogenic process” at Dept of Pharmaceutical Sciences, University of Padova, Italy
2007: Short term Research fellowship in "Flow Cytometric characterization of amniotic stem cells” at Dept of Pharmaceutical Sciences, University of Padova, Italy
2008: Research fellowship in "Flow Cytometric Study support in Polesine for IOV project on LLC” at ULSS18 – Rovigo, Italy
2008-present: Assistant Professor of BIO16
Teaching
Office hours
Friday, only on request
Availability for Thesis Projects
from October 2016
Research
Scientific Activities
Isolation of stem cells/progenitors from human peripheral blood by High Gradient Magnetic Cell Separation (MACS) and Fluorescence activated cell sorting (FACS) for tissue engineering applications, establishment of in vivo models for testing the regenerative potentialities of circulating multipotent stem cells, immunophenotyping of glial and neuronal compartments from CNS and ENS of rat and zebrafish, study of Wnt signaling in gut inflammatory diseases, development of anti-inflammatory strategies for inhibiting the formation of silicone periprosthetic capsule, study of liver regeneration after chemical insult and biliary damage.
03/2004-09/2004 professional abroad experience on "Study of Wnt system in adipogenic differentiation” at School of Medicine - Boston University (USA)
From 2007 member of Italian Society of flow cytometry (GIC); from 2014 member of Local Ethics Committee (OPBA)-University of Padova (Italy); from 2015 member of the American Physiological Society (APS)
Technical expertise
Cell biology and tissue engineering techniques; isolation and immunophenotyping of multipotent stem cells from human adipose tissue, Warthon jelly, umbilical cord blood, human peripheral blood; isolation, culture and functional characterization of preadipocytes, condroblasts, osteoblasts, endothelial cells from human and animal samples; expertise in flow cytometric analysis by BD FACSCalibur TM, BD FACSCanto II flow cytometer, cell sorting by MoFlo high speed cell sorter from Beckman Coulter (originally Cytomation); PCR NAT based test by Roche CobasS 201; DNA sequencing by ABI PRISM 377 DNA SEQUENCER, spectratyping by ABI PRISM 310 Genetic Analyzer; production of recombinant proteins, gene silencing by siRNA; cytokine profiling by Multiplexed Bead-Based Immunoassays; study of Wnt, NF-kB and BMP signaling pathways; confocal microscopy image analysis by Leica TCS SP5; acquired experience in one-step RT-PCR and qPCR.
Publications
- Di Liddo R, Valente S, Taurone S, Zwergel C, Marrocco B, Turchetta R, Conconi MT, Scarpa C, Bertalot T, Schrenk S, Mai A, Artico M. Histone deacetylase inhibitors restore IL-10 expression in lipopolysaccharide-induced cell inflammation and reduce IL-1β and IL-6 production in breast silicone implant in C57BL/6J wild-type murine model. Autoimmunity. 2016 Jan 20:1-11. [Epub ahead of print] PubMed PMID: 26789595. (first author, IF 2.714, Q3)
- Di Liddo R, Bertalot T, Schuster A, Schrenk S, Muller O, Apfel J, Reischmann P, Rajendran S, Sfriso R, Gasparella M, Parnigotto PP, Conconi MT, Schäfer KH. Fluorescence-Based Gene Reporter plasmid to track canonical Wnt signalling in ENS inflammation. Am J Physiol Gastrointest Liver Physiol. 2016 Jan 14:ajpgi.00191.2015. doi: 10.1152/ajpgi.00191.2015. [Epub ahead of print] PubMed PMID: 26767983. (first author, IF 3.798, Q1)
- Marinelli C, Di Liddo R, Facci L, Bertalot T, Conconi MT, Zusso M, Skaper SD, Giusti P. Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes. J Neuroinflammation. 2015 Dec 30;12(1):244. doi: 10.1186/s12974-015-0458-6. PubMed PMID: 26714634; PubMed Central PMCID: PMC4696218. (co-first author, IF 5.408, Q1)
- Di Liddo R, Bertalot T, Schuster A, Schrenk S, Tasso A, Zanusso I, Conconi MT, Schäfer KH. Anti-inflammatory activity of Wnt signaling in enteric nervous system: in vitro preliminary evidences in rat primary cultures. J Neuroinflammation. 2015 Feb 3;12:23. doi: 10.1186/s12974-015-0248-1. PubMed PMID: 25644719; PubMed Central PMCID: PMC4332439. (first author, IF 5.408, Q1)
- Di Liddo R, Grandi C, Dalzoppo D, Villani V, Venturini M, Negro A, Sartore L, Artico M, Conconi MT, Parnigotto PP. In vitro evaluation of TAT-OP1 osteogenic properties and prospects for in vivo applications. J Tissue Eng Regen Med. 2014 Sep;8(9):694-705. doi: 10.1002/term.1568. Epub 2012 Sep 13. PubMed PMID: 22972614.(first author, IF 5.199, Q1)
Patents
01/07/10 - 20100003223 -Method for the production of multicomponent stem cells, relative kits and uses in the medical field, international patent
Research projects and Funds
2009-2015: Ex 60% Projects, University of Padova;
2009: Research Grant, University of Padova
Isolamento e caratterizzazione di cellule staminali VSEL da sangue periferico umano per applicazioni di ingegneria tessutale
2012: Research Project of Athenaeum, University of Padova
Novel histone deacetylase inhibitors to obtain pluripotent stem cells from human fibroblasts
2013; Research Grant, University of Padova
Cellule staminali multipotenti da sangue periferico umano per la rigenerazione del miocardio
2015; Financial support from Steigerwald Arzneimittelwerk GmbH (Darmstadt, Germany)
STW5 and Wnt signaling in gut inflammation
