Nanobodies or camelid antibody fragments: properties and applications - Prof. Dumoulin

Dal 10.11.2025 al 12.11.2025

Nanobodies or camelid antibody fragments: properties and applications

Mireille Dumoulin, Ph.D

 NEPTUNS, Nanobodies to Explore Protein Structure and Functions, Centre for Protein Engineering,

 InBIOS, University of Liège, Belgium

Place: Department of Pharmaceutical and Pharmacological Sciences - Aula:6

Info: patrizia.polverinodelaureto@unipd.it

To enroll:  https://medicina.elearning.unipd.it/course/view.php?id=7255

Lecture 1,

1- What are Nanobodies? This part will explain how Nanobodies were discovered and give an overview of the unique properties of Nanobodies compared to those of conventional antibody fragments such as Fab, and ScFv.

2- How Nanobodies are generated and characterized? This lecture will introduce the different steps involved to generate and select Nanobodies against a given antigen, and to characterize them in term of affinity, specificity, epitope mapping, stability using various a range of technics including ELISA, SPR, Biolayer interferometry, ITC, fluorescence and circular dichroism

Lecture 2,

How to engineer Nbs to further adapt their properties to the applications: creation of multivalent/multispecific Nanobodies, creation of chimeric Nbs (fusion with toxins, enzymes, etc.). We will discuss protein engineering strategies to improve the pharmacokinetics of Nbs and to label them for in vivo imaging.

Therapeutic, diagnostic and biotechnological application of Nanobodies, part 1

Lecture 3,

Therapeutic, diagnostic and biotechnological applications of Nanobodies, part 2