Short courses Prof. Thelen - Prof. Fregno
Seminar I - Prof. Marcus Thelen
23 November 2023 h 14:00-16.00 (Room Zancan)
Role of Chemokines in Cancer
What are chemokines? A little bit of nomenclature. Structure of chemokines and chemokine receptors. Role of chemokines in cell proliferation, cell survival with particular attention on their function in cell migration. Detailed description of the cutting edge methodological approaches to study these important molecules.
Seminar II - Prof. Marcus Thelen
24 November 2023 h 09.30-11.30 (Room Zancan)
Role of Chemokines in Cancer
Role of chemokines in cancer, role in metastasis and in the tumor ability to evade the immunological surveillance. Modulation of chemokine activity as potential anticancer therapeutic strategy. The very delicate issue of chemokines modulation
Seminar I - Prof. Ilaria Fregno
06 December 2023 h 14.00-16.00 (Room Zancan)
Catabolic control of Endoplasmic reticulum homeostasis
The endoplasmic reticulum (ER) is the major membranous system of eukaryotic cell, deputed to protein production, lipid synthesis and calcium storage. The ER is a plastic organelle whose size and function can be dynamically adapted according to the cell need. Here, we will deeply discuss the selective degradation of the ER within the lysosomal compartment, a process defined as ER-phagy. A particular focus will be given to RecovER-phagy, the process that delivers the excess ER generated during the stress phase to lysosomal compartments for clearance. Experimental procedures introduced during the seminar: mammalian cell culture, immunofluorescence analysis, cell lysis and WB analysis, CRISPR-Cas9 technology, molecular dynamics simulation, Nuclear magnetic resonance, Immunogold Electron Microscopy.
Seminar II - Prof. Ilaria Fregno
07 December 2023 h 11.00-13.00 (Room Zancan)
Catabolic control of Endoplasmic reticulum homeostasis
The ER produces about 40% of the total cell proteome. Polypeptides that do not attain their native structure are recognized by the ER quality control system, translocated across the ER membrane and degraded by cytosolic 26S proteasomes via ER-associated degradation (ERAD). ERAD-resistant polypeptides are segregated in ER subdomains and delivered to endolysosomes under the control of “ER-phagy receptors”. These processes are collectively defined as ER-to-lysosome associated degradation (ERLAD). ERLAD of the Z variant of alpha one antitrypsin (ATZ) will be widely discussed. Experimental procedures introduced during the seminar: cell culture, immunofluorescence analysis, cell lysis and WB analysis, CRISPR-Cas9 technology, Immunogold Electron Microscopy, Halo-tag pulse/chase
