Chiara Bolego
 | Department of Pharmaceutical and Pharmacological Sciences, University of Padova Contacts |
Short CV
EDUCATION and RESEARCH EXPERIENCE
1998-2007 Research Associate, Department of Pharmacological Sciences, University of Milan
1998 Research Fellow,Institute of Arteriosclerosis Research, University of Münster, Germany
1997 PhD in Pharmacology of Natural Compounds, University of Milan
1994 Graduate School in Pharmacology, University of Milan
1992 Research Fellow, Walter Straub Institute of Pharmacology University of Munich, University of Munich
1991 Master Degree in Pharmacy, University of Padua
FELLOWSHIPS/AWARDS
1991-1992: Winner of a grant from the University of Padova for a fellowship at the Institute of Pharmacology in Munich, Germany
1992-1993: Winner of a fellowship from the University of Milan to attend the Graduate School in Pharmacology
1997: Winner of a grant from the Nutrition Foundation of Italy
1998: Fellowship at the Institute of Arteriosclerosis Research, University of Münster, Germany
1998-1999: Post-doctoral fellowship, University of Milan
2001-2002: Winner of a fellowship from the Nutrition Foundation of Italy
1998: Best abstract prize, 70th Congress of the European Atherosclerosis Society
Travel grants from the Italian Pharmacological Society to attend the 38th and 39th National Congresses
2003-2006: Post-doctoral research fellowships from the Institute of Pharmacological Sciences, University of Milan
2006-2007: Post-doctoral research fellowship funded by the EICOSANOX Consortium.
POSITIONS AND EMPLOYMENT
2014-present Associate Professor of Pharmacology, Department of Pharmaceutical and Pharmacological Sciences, University of Padua
2007- 2014 Assistant Professor of Pharmacology, Department of Pharmacology, University of Padova
February-August 2010: Visiting Scientist, Diabetes and Obesity Center of Excellence, Seattle, WA, USA
2017: Abilitazione Scientifica Nazionale for Full Professor sector 05/G1 PHARMACOLOGY, CLINICAL PHARMACOLOGY AND PHARMACOGNOSYEDITORIAL ACTIVITY
OTHER WORK EXPERIENCE
2001 Scientific Consultant for the Nutrition Foundation of Italy, Milan
2000 Clinical Pharmacist, Fatebenefratelli Hospital, Milan, Italy
EDITORIAL ACTIVITY
Reviewer for several international Journals. Member of PLOs ONE and Frontiers in Pharmacology Editorial board
SCIENTIFIC ACTIVITIES
Member of the Teaching Board of the PhD Program in Pharamcological Sciences (2009-present)
Member of the University of Padova Research Council-Pharamceutical Sciences (2021-present)
Member of the University of Padova Open Science Commitee (2022-present)
Director of the post-graduate Course in Clinical Pharamcy and Pharamcology (2014-2016)
Grant Application Reviewer fro the French National Research Agency
TEACHING ACTIVITY
2021-present: Teaching Cardiovascular Pharamcology, Master Degree in Medicine and Surgery
2022 ongoing: Teaching Pharmacology and Molecular Therapies (english), Master Degree in Medical Biotechnologies
2018-present: Teaching Pharamcogenetics and principles of personalized medicine, Graduate School in Hospital Pharmacy
2013-2021: Teaching Pharmacogenetics and Pharmacogenomics, Master Degree in Pharmacy
2007-present:Teaching Molecular Pharmacology, Master Degree in Pharmaceutical Biotechnology (english)
1998 and 2001: Contract Professor, Postgraduate School of Pharmacology, University of Milan
RESEARCH ACTIVITY
Author of 73 publications in international, peer-reviewed journals (h index: 31)
2 book chapters; 2 Congress Proceedings
Teaching
Office hours
to be determined by e-mail
Availability for Thesis Projects
Effects of estrogens on endothelial cell metabolism and angiogenesis; effects of estrogens on macrophage immunophenotypes and function; pharmacological modulation of eicosanoids and metabolites generated by aspirin-acetylated COX-2.
Research
Scientific Activities
The research of my laboratory is mainly in the field of cardiovascular pharmacology.
General objectives:
- Function and pharmacological regulation estrogen signaling, hormone replacement therapy and gender medicine - estrogenic pathways in diabetes-associated vascular dysfunction and inflammation, role of selective estrogen receptor modulators in complex organ and in vivo animal models
- Pharmacological modulation of the lipid mediator (eicosanoids) profile in human endothelial and innate immunity (monocyte-derived macrophages) cells in the setting of chronic inflammatory diseases - expression and regulation of biosynthetic enzymes and receptors; cell-cell interactions as a fundamental mechanism of local synthesis; pre-clinical characterization of novel pharmacological tools
Focus of ongoing research
- Sex and estrogen signaling to control inflammaging
- Functional characterization of next generation NSAIDs namley COX-2/thromboxane receptor inhibitors
Technical expertise
Primary cell culture models: isolation of monocyte-derived and mouse bone marrow-derived macrophages; isolation of human umbilical vein endothelial cell (HUVEC) and co-culture protocols;
In vivo studies: diabetes induction, ovariectomy, castration, in vivo replacement with osmotic pumps, gavage and other in vivo procedures
Ex vivo studies: isolated preparations of different tissues (in particular blood vessels from rodents)
In vitro functional assays of angiogenesis and transedothelial migration (permeability, migration, tube-like formation, invasion)
Specific techniques: flow cytometry (immunophenotypic characterization of macrophage subtypes, cytokine and ROS production); Western blot (signaling proteins involved in inflammation and oxidative stress); ELISA; immunoprecipitation of target proteins; RT-PCR and real time PCR; basic histological methods (vessel IHC); immunocytochemistry including confocal microscopy analysis
Publications
- Baggio C, Ramaschi GE, Oliviero F, Ramonda R, Sfriso P, Trevisi L, Cignarella A, Bolego C. Sex-dependent PD-L1/sPD-L1 trafficking in human endothelial cells in response to inflammatory cytokines and VEGF. Biomedicine and Pharmacotherapy, 2023 Accepted.
- Boscaro C, Trenti A, Baggio C, Scapin C, Trevisi L, Cignarella A, Bolego C. Sex Differences in the Pro-Angiogenic Response of Human Endothelial Cells: Focus on PFKFB3 and FAK Activation. Front Pharmacol. 2020; 17;11:587221
- Boscaro C, Carotti M, Albiero M, Trenti A, Fadini GP, Trevisi L, Sandonà D, Cignarella A, Bolego C. Non-genomic mechanisms in the estrogen regulation of glycolytic protein levels in endothelial cells. FASEB J. 2020;34(9):12768-12784.
- Carnevali S, Buccellati C, Bolego C, Bertinaria M, Rovati GE, Sala A. Nonsteroidal Anti-Inflammatory Drugs: Exploiting Bivalent COXIB/ TP Antagonists for the Control of Cardiovascular Risk. Curr Med Chem. 2017;24(30):3218-323
Research projects and Funds
2022 PRID Pharmacological characterization of Toll-like receptor 4 as a novel target for opioids in central and peripheral immune cells: implications for improving chronic pain management. Dipartimento di Scienze del Farmaco Università di Padova.Ruolo:co-investigator
2018 PRID Identification of novel functional and molecular targets for cardiac glycosides beyond the heart Na/K ATPase: focus on estrogen-dependent cancer inflammatory microenvironment. Dipartimento di Scienze del Farmaco Università di Padova. Ruolo:PI
2017 Meccanismi potenziali di reazioni averse da uso concomitante di integratori nel paziente oncologico: studio del potenziale infiammatorio in monociti umani, finanziato dal CRO di Aviano. Ruolo: PI
2013: supervisor of the Graduate Fellowship grant CPDR130758/13 "Activation phenotypes and lipid mediator signature in human monocyte-derived macrophages"
2014-2016 Natural modulators of microglia and macrophage polarization. University of Padua PRAT CPDA144389. Ruolo: Co-investigator
2008-2010 Effects of in vivo administration of selective estrogen receptor agonists in rats on multiple parameters of cardiovascular and uterine function. University of Padua. PRAT CPDA082973. Ruolo: PI
